OmniProt™

OmniProt™ Elevates Your Research!

OmniProt™ is a group of high-performance beads/nanoparticles, which offers a better way to enrich low-abundance proteins, improving sensitivity and reproducibility in the preparation of plasma/serum samples for proteomic mass spectrometry analysis.

OmniProt™ workflow

OmniProt™ is ideal for lab - scale experiments and rapid tech development. Its flexible design allows easy customization of each step, enabling precise research and fast-paced innovation without rigid protocols. A reference protocol is provided below, and we eagerly anticipate your laboratory developing even more efficient protocols.

OmniProt™ working principle

· Using a Scanning Electron Microscope (SEM) to view the enrichment：

1. Original nanoparticles are uniformly spherical with a radius of about 441-464 nm.

2. After protein binding, nanoparticles develop a dynamic protein corona, increasing the radius to approximately 467-532 nm.

· Dynamic protein corona - powered selective protein enrichment：

OmniProt™ uses nanoparticles that form a dynamic protein corona when exposed to plasma or serum, creating a biological interface that enables selective enrichment of proteins across various abundance levels. [1]

By engineering a protein corona through nanoparticle-plasma interactions, OmniProt™ significantly enhances the identification of low- and medium-abundance proteins in human and mouse plasma/serum samples.

Baize will tell the truth！

To ensure data quality and interpretability in NP-based plasma proteomics, researchers at Westlake University developed an open-access software, Baize, for evaluating sample contamination caused by platelets, erythrocytes, and coagulation-related proteins.

https://www.guomics.com/Baize/

More details

[1] A comprehensive evaluation of OmniProt's performance has been published in a preprint on bioRxiv. We invite the community to review the detailed analysis and share feedback for improvement. Read the article.