OmniProt™ Elevates your research!
OmniProt™ is a group of high-performance nanoparticle, which offers a robust way to enrich low-abundance proteins, improving sensitivity and reproducibility in the preparation of plasma/serum samples for proteomic mass spectrometry analysis.
High-abundance protein removal
Low-abundance protein enrichment
Mass spectrometry compatibility
Sensitivity and reproducibility
OmniProt™ workflow
OmniProt™ is ideal for lab-scale experiments and rapid tech development. Its flexible design allows easy customization of each step, enabling precise research and fast-paced innovation without rigid protocols. A reference protocol is provided below. [1]
①Sample
Mammalian plasma/serum samples (human or mouse).
②Enrichment
Incubate samples with OmniProt™ beads at 30 ℃ for 30 min to form protein corona.
③Lysis
Remove excess proteins, then lyse, reduce and alkylate to prepare proteins for digestion.
④Digestion
Digest proteins into peptides using trypsin.
⑤Cleanup
Desalt and purify peptide mixtures before LC-MS analysis.
⑦MS Analysis
Run LC-MS/MS using TMT (30 fractions) or Astral DlA (60 samples/day).
OmniProt™ working principle
· Using a Scanning Electron Microscope (SEM) to view the enrichment：
1. Original nanoparticles are uniformly spherical with a radius of about 441-464 nm.
2. After protein binding, nanoparticles develop a dynamic protein corona, and increase the radius to approximately 467-532 nm.
· Dynamic protein corona - powered selective protein enrichment：
OmniProt™ uses nanoparticles that form a dynamic protein corona when exposed to plasma or serum, creating a biological interface that enables selective enrichment of proteins across various abundance levels. [1]
By engineering a protein corona through nanoparticle-plasma interactions, OmniProt™ significantly enhances the identification of low- and medium-abundance proteins in human and mouse plasma/serum samples.
OmniProt™ technical approaches & performance
· Application of OmniProt™ to lung cancer cohorts
OmniProt™ is optimized for low-abundance protein detection in plasma/serum, delivering:
220% improved peptide ID
260% enhanced protein discovery
（Using Astral DIA 60SPD）
· Comparison of plasma proteomics technologies
Compared to untreated human plasma. OmniProt™ enrichment increased low-abundance protein identification by 3x.
Compared to High Select Top14 Kit（Thermo Fisher Scientific）-treated plasma, OmniProt™ boosted identification of low-and medium-abundance proteins by 4.5x.
（Using Astral DIA 60SPD）
Baize will tell the truth！
To ensure data quality and interpretability in NP-based plasma proteomics, researchers at Westlake University developed an open-access software, Baize, for evaluating sample contamination caused by platelets, erythrocytes, and coagulation-related proteins.
https://www.guomics.com/Baize/
More details
[1] A comprehensive evaluation of OmniProt's performance has been published in a preprint on bioRxiv. We invite the community to review the detailed analysis and share feedback for improvement. Read the article.
Get In Touch !
E-mail: RC_sales@westlakeomics.com
Tel: +86 571-86780630
Web: www.westlakeomics.com
