PRM/MRM

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Introduction

MRM (Multiple Reaction Monitoring) is a targeted mass spectrometry acquisition mode, and the mainstream method in this regard, commonly used for relative or absolute quantification of peptides or other small molecules. With a wide linear dynamic range, and excellent sensitivity, accuracy, and reproducibility, even a high specificity in the presence of chemical standards, MRM is mainly achieved by triple quadrupole mass spectrometry, and PRM is supported by Orbitrap instruments.

Application Scenarios: Absolute quantification of peptides (with external standards or with stable-isotope labeled internal standards)

Workflow:

Mass Spectrometers

MRM: Sciex TripleTOF 5600, Sciex 4500 MD

SRM: Thermo Scientific Altis

PRM: Thermo Scientific Q Exactive

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